Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow NOS2 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • VEZF1 elisa kit blog
 • SERPINC1 elisa kit blog
 • ELOA elisa kit blog
 • TMOD3 elisa kit blog
 • CXCR1 elisa kit blog
 • MYOM2 elisa kit blog
 • KLK6 elisa kit blog
 • HSF2 elisa kit blog
 • PA/PLB clia kit blog
 • MRP1 elisa kit blog
 • SEMA3B elisa kit blog
 • CSPG4 elisa kit blog
 • ACO elisa kit blog
 • BTG elisa kit blog
 • ZNF528 elisa kit blog
 • IL-4 elisa kit blog
 • 11-KT elisa kit blog
 • C3bR elisa kit blog
 • ARRbeta2 elisa kit blog
 • CRLF3 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • KCNG4 Antibody
 • Synuclein (SNCA) Antibody
 • CFHR1 Antibody
 • aldo-keto reductase family 1, member C1 (dihydrodiol dehydro... ELISA Kit
 • RAB20 Antibody
 • MyD88 Antibody
 • NADPH oxidase 3 (NOX3) ELISA Kit
 • SIX4 Antibody
 downarrow more ...
separator

NOS2 elisa kit product blog

separator
Posted on 2016-02-03 10:48:12 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Rabbit ELISA Kit; NOS2; NOS2 elisa kit; Nitric Oxide Synthase 2, Inducible (NOS2);
separator
The Rabbit NOS2 n/a (Catalog #MBS2022101) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2022101 ELISA Kit recognizes Rabbit (General) NOS2.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) NOS2.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase 2, Inducible (NOS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase 2, Inducible (NOS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase 2, Inducible (NOS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Synthase 2, Inducible (NOS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Type: Double-antibody Sandwich
Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range: 0.156-10ng/mL
Sensitivity: < 0.055ng/mL. Application: Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitric Oxide Synthase 2, Inducible (NOS2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitric Oxide Synthase 2, Inducible (NOS2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =: SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%. Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions