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NGAL elisa kit product blog

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Posted on 2017-07-11 13:38:06 by mybiosource_staff
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Tags: ELISA Kit; Rat ELISA Kit; Neutrophil Gelatinase Associated Lipocalin; NGAL elisa kit; NGAL;
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The Rat NGAL n/a (Catalog #MBS059267) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS059267 ELISA Kit recognizes Rat (Rattus, General) NGAL.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NGAL concentrations in Rat serum, plasma and other body fluids. Using Purified Rat NGAL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NGAL and NGAL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NGAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NGAL n/a

Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type: Sandwich
Detection Range: 15.6 ng/ml - 500 ng/ml.
Sensitivity: 2.0 ng/ml. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NGAL concentrations in Rat serum, plasma and other body fluids. Using Purified Rat NGAL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NGAL and NGAL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NGAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision: Intra-assay CV (%) is less than 15%.
Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]. Rat Neutrophil Gelatinase Associated Lipocalin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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