|The Rat NFkB n/a (Catalog #MBS2000412) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2000412 CLIA Kit recognizes Rat (Rattus, General) NFkB.
The NFkB n/a product has the following accession number(s) (GI #525314262) (NCBI Accession #NP_001266238.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the Nuclear Factor Kappa B (NFkB) CLIA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
The microplate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Kappa B (NFkB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nuclear Factor Kappa B (NFkB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate An and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nuclear Factor Kappa B (NFkB) level in the sample or standard.;.
Assay Type: Double-antibody Sandwich
Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range: 27.4-20,000pg/mL
Sensitivity: < 12.5pg/mL. Application: Chemiluminescent immunoassay for Antigen Detection.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Factor Kappa B (NFkB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Factor Kappa B (NFkB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =: SD/meanX100