Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow NE elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • DEFB128 elisa kit blog
 • MBNL2 elisa kit blog
 • enin alpha-1 (CTNNA1) elisa kit blog
 • COL11a1 elisa kit blog
 • MMP-9 elisa kit blog
 • PLG elisa kit blog
 • TLR8 elisa kit blog
 • GSTalpha5 elisa kit blog
 • MAP6D1 elisa kit blog
 • ZC3H12A elisa kit blog
 • AFP-L2 elisa kit blog
 • CDC42BPG elisa kit blog
 • PALB A elisa kit blog
 • HMGA1 elisa kit blog
 • DRD1 elisa kit blog
 • ETFDH elisa kit blog
 • MECP2 elisa kit blog
 • INB elisa kit blog
 • USO1 elisa kit blog
 • MT-ND4L elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • pro-gastrin-releasing peptide, ProGRP ELISA Kit
 • MMP-17 ELISA Kit
 • V (D)J recombination-activating protein 1 (RAG1) Recombinant Protein
 • ACE Purified Protein
 • Klebsiella pneumoniae PCR Kit
 • Angiotensin II (ANG II) ELISA Kit
 • E-Cadherin (CDH1) Antibody
 • L-2-hydroxyglutarate dehydrogenase, mitochondrial (L2HGDH) Recombinant Protein
 • POLR2F Antibody
 • Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) ELISA Kit
 • Von Hippel-Lindau Protein (VHL) Recombinant Protein
 • Progesterone Receptor (PRGR) Antibody
 • Plakoglobin (JUP) Antibody
 downarrow more ...

NE elisa kit product blog

Posted on 2015-07-28 08:09:54 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Bovine ELISA Kit; Neutrophil Elastase; NE elisa kit;
The Bovine NE elane (Catalog #MBS740418) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS740418 ELISA Kit recognizes Bovine (Cow, Cattle, General) NE.

The NE elane product has the following accession number(s) (GI #4503549) (NCBI Accession #NP_001963.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Bovine Neutrophil Elastase ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) NE.

Principle of the assay: NE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NE antibody and an NE-HRP conjugate. The assay sample and buffer are incubated together with NE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NE concentration since NE from samples and NE-HRP conjugate compete for the anti-NE antibody binding site. Since the number of sites is limited, as more sites are occupied by NE from the sample, fewer sites are left to bind NE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NE concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 pg/mL. Intended Uses: This NE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine NE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Bovine Neutrophil Elastase ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Cyclic neutropenia, Disease Models, Animal, Inflammation, Liver Diseases, Lung Diseases, Neutropenia, Neutropenia, Severe Congenital, Autosomal Dominant 1, Pain, Shock, Weight Loss are some of the diseases may be linked to Bovine Neutrophil Elastase ELISA Kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions