Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow NAGA elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • AP/3A-5A-THP elisa kit blog
 • PGM elisa kit blog
 • gp-39 elisa kit blog
 • MACC1 elisa kit blog
 • PON elisa kit blog
 • INHA elisa kit blog
 • LP-PL-A2 elisa kit blog
 • SP1 elisa kit blog
 • PLCD4 elisa kit blog
 • Anti-KIF20B elisa kit blog
 • AKR1C3 elisa kit blog
 • ASK1 clia kit blog
 • SOX3 elisa kit blog
 • GRIK2 elisa kit blog
 • TGFb1 elisa kit blog
 • ABCA13 elisa kit blog
 • MIS/AMH elisa kit blog
 • D-LDH elisa kit blog
 • GPI elisa kit blog
 • C3AR1 elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Lipoarabinomannan (LAM) ELISA Kit
 • 50S ribosomal protein L34 Recombinant Protein
 • Endothelin 1 (ET-1) ELISA Kit
 • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase,... ELISA Kit
 • E. coli (O & K antigens) Antibody
 • Glutathione S-transferase class-mu 26 kDa isozyme (GST 26) Recombinant Protein
 • ERCC8 Antibody
 • Catecholamine ELISA Kit
 • PCNA Antibody
 • Total Bilirubin (TBIL) ELISA Kit
 • Phosphinothricin N-acetyltransferase (bar) Recombinant Protein
 • NSE ELISA Kit
 • nerve growth factor (beta polypeptide) (NGF) ELISA Kit
 • D-3-phosphoglycerate dehydrogenase, PHGDH ELISA Kit
 downarrow more ...
separator

NAGA elisa kit product blog

separator
Posted on 2015-12-07 11:53:33 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; NAGA; NAGA elisa kit; Alpha N acetylgalactosaminidase (NAGA);
separator
The Human NAGA naga (Catalog #MBS7214609) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS7214609 ELISA Kit recognizes Human (General) NAGA.

The NAGA naga product has the following accession number(s) (GI #4557781) (NCBI Accession #NP_000253.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Alpha N acetylgalactosaminidase (NAGA) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) NAGA.

Principle of the assay: NAGA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NAGA antibody and an NAGA-HRP conjugate. The assay sample and buffer are incubated together with NAGA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NAGA concentration since NAGA from samples and NAGA-HRP conjugate compete for the anti-NAGA antibody binding site. Since the number of sites is limited, as more sites are occupied by NAGA from the sample, fewer sites are left to bind NAGA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NAGA concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Intended Uses: This NAGA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NAGA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Human Alpha N acetylgalactosaminidase (NAGA) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. NAGA also interacts with the following gene(s): ACO2, HEXA, HEXB, M6PR, NANH. The following patways have been known to be associated with this gene.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions