|The Human MSLN msln (Catalog #MBS8244581) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS8244581 ELISA Kit recognizes Human (General) MSLN. MyBioSource\'s Mesothelin can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the MSLN msln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MSLN msln product has the following accession number(s) (GI #116242654) (NCBI Accession #Q13421.2) (Uniprot Accession #Q13421). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Mesothelin ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve
Principle of the Assay: The Human Mesothelin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Mesothelin in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Human Mesothelin coated on a 96-well plate. Standards and samples are pipetted into the wells and Mesothelin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Mesothelin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mesothelin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.
Samples: Cell Culture Supernatants, Serum, Plasma, Urine.
Sensitivity: 10 pg/ml. Human Mesothelin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.