|The Human MMP14 mmp14 (Catalog #MBS178524) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS178524 ELISA Kit recognizes Human (General) MMP14.
The MMP14 mmp14 product has the following accession number(s) (GI #4826834) (NCBI Accession #NP_004986.1) (Uniprot Accession #P50281). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human MMP-14 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) (Human MMP-14 PicoKine ELISA Kit standard curve )
Principle of the Assay: Human MMP-14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-14 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A21-S538) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MMP-14 amount of sample captured in plate.
Background/Introduction: Matrix metalloproteinase-14 is an enzyme that in humans is encoded by the MMP14 gene. It is mapped to chromosome 14q11-q12. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP\'s are secreted as inactive pro-proteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted.
Samples: Cell culture supernates, serum and plasma(heparin).
Assay Type: Sandwich
Detection Range: 156pg/ml-10000pg/ml
Sensitivity: <15pg/ml. Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Human MMP-14 PicoKine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. MMP14 also interacts with the following gene(s): ADI1, FURIN, LUM, MMP2, SP1, TIMP1, TIMP2. Blood, Bone, Brain, Connective Tissue, Embryonic Tissue, Heart, Lung, Muscle, Skin, Vascular tissues are correlated with this protein. Adenocarcinoma, Breast Neoplasms, Cardiovascular Diseases, Disease Models, Animal, Inflammation, Lung Diseases, Lung Neoplasms, Neoplasm Invasiveness, Neoplasm Metastasis, Neoplasms, Experimental are some of the diseases may be linked to Human MMP-14 PicoKine ELISA Kit. The following patways have been known to be associated with this gene.