Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow MMP14 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • MUC5AC elisa kit blog
 • PRO-HEPC elisa kit blog
 • LUM elisa kit blog
 • PRL elisa kit blog
 • CCDC41 elisa kit blog
 • CRLF1 elisa kit blog
 • CCK-8 elisa kit blog
 • GPRC5A elisa kit blog
 • ABP elisa kit blog
 • CRIP1 elisa kit blog
 • MAFG elisa kit blog
 • CTNNA1 elisa kit blog
 • EP elisa kit blog
 • TRH elisa kit blog
 • HCRTR2 elisa kit blog
 • DNMT3B elisa kit blog
 • betaGD elisa kit blog
 • PRL elisa kit blog
 • MAPKAPK3 elisa kit blog
 • DDX1 elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • OPRM1 - MOR1 Antibody
 • Leptin Antibody
 • Glucoamylase Enzyme
 • SLURP1 Antibody
 • CD20 Antibody
 • ANXA2R Antibody
 • IL-13 ELISA Kit
 • ERK3 (Phospho-Ser189) Antibody
 • Lipopolysaccharide-binding protein (LBP) ELISA Kit
 • Sirtuin 3 (SIRT3) ELISA Kit
 • Glutenin, high molecular weight subunit DX5 (GLU-1D-1D) Recombinant Protein
 • FASLG Antibody
 • Chromatin Assembly Factor 1, p150 (Caf1) Antibody
 • Leptin Receptor (LEPR) Peptide
 downarrow more ...

MMP14 elisa kit product blog

Posted on 2018-06-13 08:10:29 by mybiosource_staff
Share it on ...     
Tags: MMP14; ELISA Kit; Human ELISA Kit; MMP14 elisa kit;
The Human MMP14 mmp14 (Catalog #MBS178524) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS178524 ELISA Kit recognizes Human (General) MMP14.

The MMP14 mmp14 product has the following accession number(s) (GI #4826834) (NCBI Accession #NP_004986.1) (Uniprot Accession #P50281). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human MMP-14 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) MMP14.

Principle of the Assay: Human MMP-14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for MMP-14 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: A21-S538) and test samples are added to the wells, a biotinylated detection specific for MMP-14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MMP-14 amount of sample captured in plate.

Background: Matrix metalloproteinase-14 is an enzyme that in humans is encoded by the MMP14 gene. It is mapped to chromosome 14q11-q12. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP\'s are secreted as inactive pro-proteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted.

Assay Type: Quantitative Sandwich
Detection Range: 156pg/ml-10000pg/ml
Sensitivity: <15pg/ml. Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. Human MMP-14 PicoKine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Bone, Brain, Connective Tissue, Embryonic Tissue, Heart, Lung, Muscle, Skin, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. Adenocarcinoma, Breast Neoplasms, Cardiovascular Diseases, Disease Models, Animal, Inflammation, Lung Diseases, Lung Neoplasms, Neoplasm Invasiveness, Neoplasm Metastasis, Neoplasms, Experimental are some of the diseases may be linked to Human MMP-14 PicoKine ELISA Kit. MMP14 also interacts with the following gene(s): ADI1, FURIN, LUM, MMP2, SP1, TIMP1, TIMP2.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions