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MLL5 elisa kit product blog

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Posted on 2015-09-26 22:38:01 by mybiosource_staff
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Tags: ELISA Kit; Canine ELISA Kit; Myeloid/lymphoid or mixed-lineage leukemia 5; MLL5 elisa kit; MLL5;
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The Canine MLL5 n/a (Catalog #MBS750269) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS750269 ELISA Kit recognizes Canine (Dog, General) MLL5.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Principle of the Assay: MLL5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MLL5 antibody and an MLL5-HRP conjugate. The assay sample and buffer are incubated together with MLL5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MLL5 concentration since MLL5 from samples and MLL5-HRP conjugate compete for the anti-MLL5 antibody binding site. Since the number of sites is limited, as more sites are occupied by MLL5 from the sample, fewer sites are left to bind MLL5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MLL5 concentration in each sample is interpolated from this standard curve.

MLL5 n/a

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 0.5-10g/mL
Sensitivity: 0.1ng/mL. Intended Uses: This MLL5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine MLL5. This ELISA kit for research use only, not for therapeutic or diagnostic applications
!Intended Uses: This MLL5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine MLL5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Canine Myeloid/lymphoid or mixed-lineage leukemia 5 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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