Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Assay Kit Blog  rightarrow MID1 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • PIGK elisa kit blog
 • NT-4 elisa kit blog
 • OSMR elisa kit blog
 • ASF elisa kit blog
 • ALDH5A1 elisa kit blog
 • Pre-APO-A1 elisa kit blog
 • INH elisa kit blog
 • GLB1L2 elisa kit blog
 • AMY1 elisa kit blog
 • NAGase clia kit blog
 • GRK4 elisa kit blog
 • SNRNP200 elisa kit blog
 • C5 elisa kit blog
 • ERK1 elisa kit blog
 • ADAMTS5 elisa kit blog
 • PG elisa kit blog
 • CHMP4B elisa kit blog
 • MRC1 elisa kit blog
 • FGF-18 elisa kit blog
 • Bcl2L elisa kit blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • GP Protein Recombinant Protein
 • phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1) ELISA Kit
 • PR Antibody
 • Semenogelin I (SEMG1) ELISA Kit
 • Muellerian-inhibiting factor (Amh) Antibody
 • Streptavidin Reagent
 • HCK Antibody
 • 17-Hydroxyprogesterone (17-OHP) Antibody
 • RBP1 Recombinant Protein
 • Granylocyte colony stimulating factor (GCSF) Antibody
 • Herpes Simplex Virus II, Glycoprotein G, aa343-649 (HS3ST3A1... Recombinant Protein
 • Glucagon Like Peptide 1 (GLP-1) ELISA Kit
 • growth differentiation factor 11 (GDF11) ELISA Kit
 • G6PD ELISA Kit
 downarrow more ...
separator

MID1 elisa kit product blog

separator
Posted on 2017-03-04 01:51:30 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Human ELISA Kit; Mid1; MID1 elisa kit; midline 1 (Opitz/BBB syndrome);
separator
The Human MID1 mid1 (Catalog #MBS941500) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS941500 ELISA Kit recognizes Human (General) MID1.

The MID1 mid1 product has the following accession number(s) (GI #4557753) (NCBI Accession #NP_000372.1) (Uniprot Accession #O15344). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Midline-1, MID1 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) MID1.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MID1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MID1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MID1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MID1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma, tissue homogenates
Assay Type: Sandwich
Detection Range: 23.44 pg/ml -1500 pg/ml.
Sensitivity: The minimum detectable dose of human MID1 is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Human Midline-1, MID1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Congenital Abnormalities, Drug Toxicity, Eye Diseases, Heart Diseases, Immune System Diseases, Neoplasms, Nervous System Diseases, Neuromuscular Diseases, OPITZ GBBB SYNDROME, X-LINKED, Prostatic Neoplasms are some of the diseases may be linked to Human Midline-1, MID1 ELISA Kit. MID1 also interacts with the following gene(s): IGBP1, MID1IP1, MID2, PPP2CB. The following patways have been known to be associated with this gene.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions