MHC-II/H-2II elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; MHC-II/H-2II elisa kit; MHC-II/H-2II;
The Mouse MHC-II/H-2II h2-ab1 (Catalog #MBS2510329) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2510329 ELISA Kit recognizes Mouse MHC-II/H-2II.The MHC-II/H-2II h2-ab1 product has the following accession number(s) (GI #199178) (NCBI Accession #AAA39541.1) (Uniprot Accession #P01921). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse MHC-II/H-2II(Major Histocompatibility Complex-II) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse MHC-II/H-2II concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MHC-II/H-2II. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MHC-II/H-2II and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MHC-II/H-2II, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MHC-II/H-2II. You can calculate the concentration of MHC-II/H-2II in the samples by comparing the OD of the samples to the standard curve.
Samples: Serum, Plasma, Biological Fluids
Assay Type: Sandwich
Detection Range: 3.125-200ng/mL
Sensitivity: Min: 1.875ng/mL; Max: 200ng/mL. Mouse MHC-II/H-2II(Major Histocompatibility Complex-II) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Blood, Bone Marrow, Heart, Intestine, Liver, Lung, Skin, Spleen, Thymus tissues are correlated with this protein.