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MGMT elisa kit product blog

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Posted on 2015-09-08 14:53:36 by mybiosource_staff
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Tags: MGMT; ELISA Kit; Mouse ELISA Kit; O-6-Methylguanine DNA Methyltransferase; MGMT elisa kit;
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The Mouse MGMT n/a (Catalog #MBS006086) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS006086 ELISA Kit recognizes Mouse (Murine, General) MGMT.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MGMT concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse MGMT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MGMT and MGMT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MGMT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

MGMT n/a

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type: Sandwich
Detection Range: 6.25U/L-200U/L.
Sensitivity: 1.0U/L. Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MGMT concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse MGMT antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MGMT and MGMT antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MGMT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision: Intra-assay CV (%) is less than 15%.
Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean 100]. Mouse O-6-Methylguanine DNA Methyltransferase ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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