Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow MG elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • SGPP1 elisa kit blog
 • JUND elisa kit blog
 • TERF2IP elisa kit blog
 • RAG-1 elisa kit blog
 • KCTD15 elisa kit blog
 • KIF3B elisa kit blog
 • BOLA1 elisa kit blog
 • KRTAP13-2 elisa kit blog
 • SOD2 elisa kit blog
 • GMCL1 elisa kit blog
 • gp130 elisa kit blog
 • DDIT4L elisa kit blog
 • ARRbeta2 elisa kit blog
 • TOMM40L elisa kit blog
 • SCD21 elisa kit blog
 • SHP-2 elisa kit blog
 • CA2 elisa kit blog
 • RLN elisa kit blog
 • GHBP elisa kit blog
 • CCL3L1 elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • TRM3 - TARBP1 Antibody
 • Early activation antigen CD69 ELISA Kit
 • TNFalpha ELISA Kit
 • MMP-13 Antibody
 • Steroid 5 Alpha Reductase (SRD5A) ELISA Kit
 • CD257 Antibody
 • YWHAZ Antibody
 • West Nile Virus E Recombinant Protein
 • Tubulin alpha-1B chain (TUBA1B) Recombinant Protein
 • chemokine (C-C motif) ligand 3 (CCL3) ELISA Kit
 • chromosome 4 open reading frame 7 (C4orf7) ELISA Kit
 • Kappa Light Chain (IGKC) Antibody
 • Parvovirus B19 VLP VP2 Recombinant Protein
 • PARP2 Recombinant Protein
 downarrow more ...

MG elisa kit product blog

Posted on 2015-11-24 22:15:33 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Rat ELISA Kit; Methylglyoxal; MG elisa kit;
The Rat MG mgsa (Catalog #MBS756085) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS756085 ELISA Kit recognizes Rat (Rattus, General) MG.

The MG mgsa product has the following accession number(s) (GI #328949861) (NCBI Accession #YP_004367196.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Methylglyoxal ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) MG.

Intended Uses: This MG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MG.

Principle of the Assay: MG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MG antibody and an MG-HRP conjugate. The assay sample and buffer are incubated together with MG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MG concentration since MG from samples and MG-HRP conjugate compete for the anti-MG antibody binding site. Since the number of sites is limited, as more sites are occupied by MG from the sample, fewer sites are left to bind MG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MG concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 ng/mL. Rat Methylglyoxal ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions