MET elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; MET; MET elisa kit; C-MET/HGFR;
The Human MET met (Catalog #MBS175804) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS175804 ELISA Kit recognizes Human MET.The MET met product has the following accession number(s) (GI #251757497) (NCBI Accession #P08581.4) (Uniprot Accession #P08581). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human C-MET/HGFR PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) (Human C-MET/HGFR PicoKine ELISA Kit standard curve )
Principle of the assay: human C-MET ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for C-MET has been precoated onto 96-well plates. Standards(NSO,E25 - R307 (alpha) &S308 - T932(beta)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for C-MET is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human C-MET amount of sample captured in plate.
Background: C-Met (MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor (HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.
Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type: Sandwich
Detection Range: 62.5pg/ml-4000pg/ml
Sensitivity: < 5pg/ml. Human C-MET/HGFR PicoKine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Adenocarcinoma, Breast Neoplasms, Carcinoma, Cardiovascular Diseases, Heart Diseases, Inflammation, Kidney Diseases, Liver Diseases, Lung Neoplasms, Schizophrenia are some of the diseases may be linked to Human C-MET/HGFR PicoKine ELISA Kit.