MDH assay kit product blog
Tags: Malate Dehydrogenase; MDH; Assay Kit; MDH assay kit;
The MDH at3g47520 (Catalog #MBS841658) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.Measurement of malate dehydrogenase activity in various tissues/cells
Analysis of citric acid cycle and malate-asparate shuttle. Researchers should empirically determine the suitability of the MDH at3g47520 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MDH at3g47520 product has the following accession number(s) (GI #15232820) (NCBI Accession #NP_190336.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Malate Dehydrogenase Activity Colorimetric Assay Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: Malate Dehydrogenase (MDH) Activity Colorimetric Assay Kit: Highly-sensitive assay to measure Malate Dehydrogenase (MDH) activity in animal tissues, adherent or suspension cells, mitochondria etc. Detection limit: < 0.5 mU. 100 assay
Background: Malate Dehydrogenase (MDH) (EC 1.1.1.37) is an important enzyme which reversibly converts L-malate into oxaloacetate in the presence of NAD. In eukaryotic cells, malate dehydrogenase has 2 isoforms: MDH1 and MDH2. MDH1 is cytosolic & participates in the malate-aspartate shuttle, which transports malate into mitochondria for utilization in ATP generation whereas MDH2 is a mitochondrial enzyme and part of the citric acid cycle. MDH activity is increased in some neurodegenerative diseases such as Alzheimer\'s disease, and abnormal MDH activity in serum can serve as a tool for severe liver damage (e.g. Hepatocellular carcinoma). In MyBioSource\'s Malate Dehydrogenase Activity Assay kit, MDH reacts with malate to form an intermediate. The generated intermediate reacts with MDH Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect less than 0.5 mU of MDH activity in various sample types.
Summary: Detection method- Absorbance (450 nm)
Applications- - Measurement of malate dehydrogenase activity in various tissues/cells - Analysis of citric acid cycle and malate-asparate shuttle.
Samples: Animal tissues such as liver, heart, muscle, etc.
Cell culture: Adherent or suspension cells
Mitochondria. Detection Method: Absorbance (450 nm)
Features & Benefits: Simple, rapid & convenient
The assay can detect Malate Dehydrogenase (MDH) Activity less than 0.5 mU in various sample types. Bud, Flower, Other, Root, Whole Plant tissues are correlated with this protein. MDH also interacts with the following gene(s): FUM1, ICDH, MLS.