|The Mouse MDC ccl22 (Catalog #MBS2025230) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2025230 CLIA Kit recognizes Mouse (Murine, General) MDC.
The MDC ccl22 product has the following accession number(s) (GI #1931581) (NCBI Accession #AAB58360.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate An and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Derived Chemokine (MDC) level in the sample or standard.
Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range: 13.72-10000pg/mL. Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Derived Chemokine (MDC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Derived Chemokine (MDC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Assay Procedure Summary: 1. Prepare all reagents, samples and standards;
2. Add 100uL standard or sample to each well. Incubate 2 hours at 37 degree C;
3. Aspirate and add 100uL prepared Detection Reagent A. Incubate 1 hour at 37 degree C;
4. Aspirate and wash 3 times;
5. Add 100uL prepared Detection Reagent B. Incubate 30 minutes at 37 degree C;
6. Aspirate and wash 5 times;
7. Add 100uL Substrate Solution. Incubate 10 minutes at 37 degree C;
8. Read RLU value immediately. MDC also interacts with the following gene(s): CCL11, CCL17, CCL2, CCL5, CCR3, CCR4, CCR5, CCR8. Blood, Bone, Eye, Kidney, Lung, Mouth, Pharynx, Skin, Thymus tissues are correlated with this protein. The following patways have been known to be associated with this gene. Cardiovascular Diseases, Dermatitis, Atopic, Hypersensitivity, Inflammation, Liver Diseases, Lung Diseases, Necrosis, Pneumonia, Respiratory Hypersensitivity, Respiratory Tract Diseases are some of the diseases may be linked to Macrophage Derived Chemokine (MDC) CLIA Kit.