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MC1R elisa kit product blog

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Posted on 2016-06-12 13:03:50 by mybiosource_staff
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Tags: ELISA Kit; Horse ELISA Kit; MC1R; MC1R elisa kit; Melanocyte-stimulating hormone receptor;
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The Horse MC1R mc1r (Catalog #MBS282544) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS282544 ELISA Kit recognizes Horse (Equine, General) MC1R.

The MC1R mc1r product has the following accession number(s) (GI #167621422) (NCBI Accession #NP_001108006.1) (Uniprot Accession #P79166). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

TUBB3 expression in several human cell lines, but not in a paclitaxel-resistant cell line. RNA interference revealed that HIF1A mediated hypoxia-induced TUBB3 expression. Chromatin immunoprecipitation analysis showed that HIF1A bound an HIF1A-binding site in the 5-prime flanking region of the TUBB3 gene. Methylation of an enhancer in the 3-prime flanking region abolished hypoxia-induced TUBB3 expression. Much lower Tubb3 levels were detected in all other adult mouse tissues examined except brain, where expression was even lower. Chromatin immunoprecipitation analysis showed that HIF1A bound an HIF1A-binding site in the 5-prime flanking region of the TUBB3 gene. Methylation of an enhancer in the 3-prime flanking region abolished hypoxia-induced TUBB3 expression.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MC1R in samples. An antibody specific for MC1R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMC1R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MC1R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MC1R bound in the initial step. The color development is stopped and the intensity of the color is measured.

MC1R mc1r

Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength: 450 nm. Horse Melanocyte-stimulating hormone receptor (MC1R) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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