MANBA elisa kit product blog
Tags: ELISA Kit; Goat ELISA Kit; MANBA elisa kit; MANBA; Beta-mannosidase;
The Goat MANBA manba (Catalog #MBS281102) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS281102 ELISA Kit recognizes Goat MANBA.The MANBA manba product has the following accession number(s) (GI #550822368) (NCBI Accession #NP_001272620.1) (Uniprot Accession #Q95327). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
MANbAL belongs to the UPF0239 family. MANBA is a member of the glycosyl hydrolase 2 family. The encoded protein localizes to the lysosome where it is the final exoglycosidase in the pathway for N-linked glycoprotein oligosaccharide catabolism. Mutations in this gene are associated with beta-mannosidosis, a lysosomal storage disease that has a wide spectrum of neurological involvement.The composite cDNA was 3,293 nucleotides, consisting of an 87-nucleotide 5-prime untranslated region, 2,640-nucleotide coding region, and 566-nucleotide 3-prime untranslated region. Analysis of a multiple tissue Northern blot demonstrated a single 3.7-kb transcript. Comparison of the caprine and bovine cDNAs revealed that 96.3% of the nucleotides and 98% of the deduced amino acids were identical.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MANBA in samples. An antibody specific for MANBA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMANBA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MANBA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MANBA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, Plasma, Other biological fluids
Assay Type: Sandwich. Sample Volume: 1-200 uL
Precision: Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength: 450 nm. Goat Beta-mannosidase (MANBA) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.