|The Human LTF/LF ltf (Catalog #MBS731129) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS731129 ELISA Kit recognizes Human (General) LTF/LF.
The LTF/LF ltf product has the following accession number(s) (GI #312433998) (NCBI Accession #NP_001186078.1) (Uniprot Accession #P02788). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Lactoferrin ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
Intended Uses: This LTF/LF ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of LTF/LF in the sample, this LTF/LF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus LTF/LF concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of LTF/LF utilizes a multiclonal anti-LTF/LF antibody and an LTF/LF-HRP conjugate. The assay sample and buffer are incubated together with LTF/LF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTF/LF concentration since LTF/LF from samples and LTF/LF-HRP conjugate compete for the anti-LTF/LF antibody binding site. Since the number of sites is limited, as more sites are occupied by LTF/LF from the sample, fewer sites are left to bind LTF/LF-HRP conjugate. Standards of known LTF/LF concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of LTF/LF. The LTF/LF concentration in each sample is interpolated from this standard curve.