LTB4 elisa kit product blog
Tags: ELISA Kit; LTB4 elisa kit; LTB4; All ELISA Kit;
The General LTB4 ltb4r2 (Catalog #MBS2515771) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2515771 ELISA Kit recognizes General LTB4.The LTB4 ltb4r2 product has the following accession number(s) (GI #9081803) (NCBI Accession #BAA99539.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the LTB4 (Leukotriene B4) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of LTB4 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LTB4. During the reaction, LTB4 in the sample or standard competes with a fixed amount of LTB4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LTB4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LTB4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 15.63-1000pg/mL
Sensitivity: 9.38pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level LTB4 were tested 20 times on one plate, respectively.
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level LTB4 were tested on 3 different plates, 20 replicates in each plate. LTB4 (Leukotriene B4) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. LTB4 also interacts with the following gene(s): LTB4R. Blood, Bone, Embryonic Tissue, Intestine, Kidney, Lung, Ovary, Pancreas, Prostate, Spleen tissues are correlated with this protein.