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LEDGF clia kit product blog

Posted on 2015-11-23 07:59:10 by mybiosource_staff
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Tags: LEDGF; CLIA Kit; Lens Epithelium Derived Growth Factor (LEDGF); LEDGF clia kit;
The LEDGF psip1 (Catalog #MBS2001242) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase.

The LEDGF psip1 product has the following accession number(s) (GI #3283352) (NCBI Accession #AAC25167.1) (Uniprot Accession #O75475). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the CLIA Kit for Lens Epithelium Derived Growth Factor (LEDGF) with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) LEDGF.

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate An and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lens Epithelium Derived Growth Factor (LEDGF) level in the sample or standard.

Assay Type: Sandwich
Samples: Tissue homogenates and other biological fluids. Detection Range: 0.014-10ng/mL The standard curve concentrations used for the ELISA\'s were 10ng/mL, 3.33ng/mL, 1.11ng/mL, 0.37ng/mL, 0.123ng/mL, 0.041ng/mL, 0.014ng/mL
Sensitivity: The minimum detectable dose of this kit is typically less than 0.065ng/mL
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lens Epithelium Derived Growth Factor (LEDGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lens Epithelium Derived Growth Factor (LEDGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Assay Procedure Summary1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37oC;
8. Read RLU value immediately.

Organism Species: Mus musculus (Mouse)
Assay Length: 4.5 hours. LEDGF also interacts with the following gene(s): HOXA9, MEN1, MLL, PABPC1, PRDX6, SP1, SRSF1. The following patways have been known to be associated with this gene. Anemia, Brain Diseases, Breast Neoplasms, Cell Transformation, Neoplastic, Death, Hematologic Diseases, Leukemia, Myeloid, Acute, Necrosis, Neoplasms, Nervous System Diseases are some of the diseases may be linked to CLIA Kit for Lens Epithelium Derived Growth Factor (LEDGF). Blood, Bone, Bone Marrow, Brain, Connective Tissue, Eye, Ganglia, Muscle, Nerve, Skin tissues are correlated with this protein.
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