KISS-54 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; KISS-54 elisa kit; KISS-54; Kisspeptin-54;
The Human KISS-54 n/a (Catalog #MBS2533433) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2533433 ELISA Kit recognizes Human KISS-54.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human KISS-54 (Kisspeptin-54) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human KISS-54 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human KISS-54. During the reaction, Human KISS-54 in the sample or standard competes with a fixed amount of Human KISS-54 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human KISS-54. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzymesubstrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Human KISS-54 in the samples is then determined by comparing the OD of the samples to the standard curve.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 62.5-4000pg/mL
Sensitivity: 37.5pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human KISS-54 were tested 20 times on one plate, respectively.
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human KISS-54 were tested on 3 different plates, 20 replicates in each plate. Human KISS-54 (Kisspeptin-54) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.