KIM-1 elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; Kim-1 elisa kit; Kim-1;
The Rat KIM-1 havcr1 (Catalog #MBS494792) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS494792 ELISA Kit recognizes Rat KIM-1.The KIM-1 havcr1 product has the following accession number(s) (GI #2665892) (NCBI Accession #AAC53546.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the KIM-1 (Rat) ELISA with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: For detection of KIM-1 in mouse serum, plasma, urine, cell culture supernatant or tissue samples. For research use only.
Principle of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-KIM-1 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-KIM-1 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the KIM-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of KIM-1 can be calculated
Background/Introduction: KIM1, also known as Hepatitis A virus cellular receptor 1, is a protein that in mouses is encoded by the HAVCR1 gene, which maps to 5q33.2. It is a major cause of orally transmitted acute hepatitis, infects primate cells, but not dog or mouse cells, after binding to the HAV cellular receptor (HAVCR). Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Khademi et al. (2004) found that differential expression of TIMs by Th1 and Th2 cells may be implicated in different phases of an autoimmune disease.
Samples: Mouse Serum, Plasma, Urine, Cell Culture Supernatant Or Tissue Samples
Assay Type: Sandwich
Detection Range: 31.2pg/ml-2000pg/ml
Sensitivity: <10pg/ml. KIM-1 (Rat) ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.