Kallikrein 3 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Kallikrein 3; Kallikrein 3 elisa kit;
The Human Kallikrein 3 n/a (Catalog #MBS494789) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS494789 ELISA Kit recognizes Human Kallikrein 3.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Kallikrein 3 ELISA with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: For quantitative detection of Fetuin An in tissue lysates or cell culture supernates. The significance of detection in human serum, plasma, body fluids is undetermined. For research use only.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Fetuin A polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Fetuin A polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Fetuin An amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Fetuin A can be calculated
Background/Introduction: Fetuin-A, also known as Alpha-2-HS-glycoprotein (AHSG) is a protein that in humans is encoded by the AHSG gene. This gene spans approximately 8.2 kb and contains 7 exons. Fetuin-A belongs to the fetuin class of plasma binding proteins and is more abundant in fetal than adult blood. It is present in the serum, and synthesized by hepatocytes. It may plays some role in the metabolism of bone because of its high affinity for calcium. Fetuin-An is a potent inhibitor of pathological calcification. In the test tube Fetuin-A can bind multiple ligands and therefore has been claimed to be involved in several functions, such as endocytosis, brain development and the formation of bone tissue.
Samples: Tissue Lysates Or Cell Culture Supernates
Assay Type: Quantitative Sandwich
Detection Range: 312pg/ml-20,000pg/ml
Sensitivity: <10pg/ml. Kallikrein 3 ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.