JCM19297_3489 assay kit product blog
Tags: Thioredoxin Reductase; Assay Kit; JCM19297_3489 assay kit; JCM19297_3489;
The Thioredoxin Reductase jcm19297_3489 (Catalog #MBS841783) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.The Thioredoxin Reductase jcm19297_3489 product has the following accession number(s) (GI #678234313) (NCBI Accession #GAK88965.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the Thioredoxin Reductase Activity Colorimetric Assay Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Product Information: Thioredoxin Reductase (TrxR) Activity Assay Kit: Convenient Colorimetric Assay for Detecting TrxR activity in Cells, Tissues etc. Rapid, Convenient & Sensitive. 100 Assays.
Background: Thioredoxin reductase (TrxR) (EC 1.8.1.9) is an ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. MyBioSource\'s Thioredoxin Reductase Assay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5\'-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a strong yellow color (lambdamax = 412 nm). Since in crude biological samples other enzymes, such as glutathione reductase and glutathione peroxidase, can also reduce DTNB, therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed: the first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.
Summary: Detection method- Absorbance (412 nm)
Species reactivity- Mammalian.
Samples: Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc. Features & Benefits: Simple procedure; takes ~ 30-40 minutes
Fast and convenient.