|The Rat ITLN1 n/a (Catalog #MBS2530769) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2530769 CLIA Kit recognizes Rat (Rattus, General) ITLN1.
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Intended Uses: This CLIA kit applies to the in vitro quantitative determination of ITLN1 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to ITLN1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ITLN1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ITLN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of ITLN1. You can calculate the concentration of ITLN1 in the samples by comparing the RLU value of the samples to the standard curve.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 62.5-4000pg/mL