Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow IRS-1 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • GRB10 elisa kit blog
 • LYPD3 elisa kit blog
 • JUNB elisa kit blog
 • Total antioxidant capacity assay kit blog
 • MGL elisa kit blog
 • OR2J3 elisa kit blog
 • FNIP1 elisa kit blog
 • ETR-B elisa kit blog
 • NUP35 elisa kit blog
 • SPJ elisa kit blog
 • SCO5352 elisa kit blog
 • IL10 elisa kit blog
 • AIPL1 elisa kit blog
 • MIF elisa kit blog
 • CACNA1G elisa kit blog
 • KLK12 elisa kit blog
 • SSFA2 elisa kit blog
 • FETU-A elisa kit blog
 • alpha1M elisa kit blog
 • 5HT elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Carbohydrate Sulfotransferase 9 (CHST9) ELISA Kit
 • c-Myb Antibody
 • PF-4708671 Inhibitor
 • PCDH10 Antibody
 • amyloid beta peptide 1-42 (Abeta1-42) ELISA Kit
 • Rat IgG Isotype Control Control & Calibrator
 • K6PP Antibody
 • Collagen Type IV, Col IV ELISA Kit
 • TyloxApol Chemical Reference
 • A Disintegrin and Metalloprotease 19 (ADAM19) ELISA Kit
 • Serine-aspartate repeat-containing protein E (sdrE), partial Recombinant Protein
 • Fibroblast Growth Factor-acidic (FGF 1) Recombinant Protein
 • TEAD3 cDNA Clone
 downarrow more ...

IRS-1 elisa kit product blog

Posted on 2016-07-29 17:05:50 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Rabbit ELISA Kit; Insulin Receptor Substrate 1; IRS-1 elisa kit; IRS-1;
The Rabbit IRS-1 irs1 (Catalog #MBS751286) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS751286 ELISA Kit recognizes Rabbit (General) IRS-1.

The IRS-1 irs1 product has the following accession number(s) (GI #5031805) (NCBI Accession #NP_005535.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Insulin receptor substrate 1 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) IRS-1.

Intended Uses: This IRS1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit IRS1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: IRS1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IRS1 antibody and an IRS1-HRP conjugate. The assay sample and buffer are incubated together with IRS1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IRS1 concentration since IRS1 from samples and IRS1-HRP conjugate compete for the anti-IRS1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IRS1 from the sample, fewer sites are left to bind IRS1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IRS1 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Rabbit Insulin receptor substrate 1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Diabetes Mellitus, Diabetes Mellitus, Type 2, Disease Models, Animal, Hyperglycemia, Hyperinsulinism, Inflammation, Insulin Resistance, Liver Diseases, Necrosis, Obesity are some of the diseases may be linked to Rabbit Insulin receptor substrate 1 ELISA Kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions