|The INS ins (Catalog #MBS355722) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.
The INS ins product has the following accession number(s) (GI #386828) (NCBI Accession #AAA59172.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Insulin with the following immunoassay(s):
Typical Testing Data/Standard Curve
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Insulin monoclonal antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-Insulin polyclonal antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Insulin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Insulin can be calculated.
Background/Introduction: Insulin, synthesized by the beta cells of the islets of Langerhans, consists of 2 dissimilar polypeptide chains, An and B, which are linked by 2 disulfide bonds. The human insulin gene contains 3 exons; exon 2 encodes the signal peptide, the B chain, and part of the C peptide, while exon 3 encodes the remainder of the C peptide and the A chain. Insulin has a potent acute antiinflammatory effect, including a reduction in intranuclear NF-kappa-B, an increase in IKB, and decreases in the generation of reactive oxygen species. It causes cells in the liver, muscle, and fat tissue to take up glucose from the blood, storing it as glycogen inside these tissues, and improved insulin-stimulated glucose uptake after endurance training results from hemodynamic adaptations as well as increased cellular protein content of individual insulin signaling components and molecules involved in glucose transport and metabolism.
Samples: Mouse serum, plasma, body fluids, tissue lysates or cell culture supernates.
Assay Type: Sandwich
Detection Range: 0 uIU/ml - 140 uIU/ml
Sensitivity: < 5 uIU/ml. Mouse Insulin or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.