IL6ST elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; IL6ST elisa kit; IL6ST; Gp130/IL6ST;
The Mouse IL6ST il6st (Catalog #MBS176494) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS176494 ELISA Kit recognizes Mouse IL6ST.The IL6ST il6st product has the following accession number(s) (GI #408360148) (NCBI Accession #Q00560.2) (Uniprot Accession #Q00560). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Gp130/IL6ST PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) (Mouse Gp130/IL6ST PicoKine ELISA Kit standard curve )
Principle of the assay: mouse Gp130 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Gp130 has been precoated onto 96-well plates. Standards(NSO,Q23 - E617) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Gp130 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Gp130 amount of sample captured in plate.
Background: GP130(Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound gp130. Gp130 is an important part of many different types of signaling complexes.Gp130 molecules with mutations in either of these segments could not transduce a growth signal.
Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Assay Type: Sandwich
Detection Range: 125pg/ml-8000pg/ml
Sensitivity: < 10pg/ml. Mouse Gp130/IL6ST PicoKine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.