|The IL17A il17a (Catalog #MBS135545) is an ELISA Kit produced from Multiple and is intended for research purposes only. The product is available for immediate purchase.
The IL17A il17a product has the following accession number(s) (GI #6754324) (NCBI Accession #NP_034682.1) (Uniprot Accession #Q62386). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse IL-17A total antigen assay ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This mouse IL-17A antigen assay is intended for the quantitative determination of total mouse IL-17A antigen in cell culture media. For research use only.
Principle of the Assay: Mouse IL-17A will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse IL-17A. The amount of color development is proportional to the concentration of mouse IL-17A in the samples.
Background: Mouse Interleukin-17A (aka IL-17, IL-17A or CTLA-8) is a 133 amino acid disulfide-linked homodimeric glycoprotein that is the founding member of the IL-17 family of proteins [1-2]. IL-17A is a proinflammatory cytokine that participates in neutrophil recruitment and is primarily expressed in CD4+ T cells . IL-17A has been shown in a mouse knockout model to play a vital role in allergen-specific immune responses via T cell activation . It binds to the IL-17RA and IL-17RC receptors which are expressed in TH17, CD8+ T, gammadeltaT, NK, NKT and LTi cells .
Samples: Cell culture media
Sensitivity: The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.056-0.069) and calculating the corresponding concentration. The MDD was 4.8 pg/ml. Intra-assay Precision: Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision: Three samples of known concentration were tested in ten independent assays to assess inter-assay precision. Mouse IL-17A total antigen assay ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.