IL13 elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; IL-13; IL13 elisa kit; IL13;
The Rat IL13 il13 (Catalog #MBS175932) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS175932 ELISA Kit recognizes Rat IL13.The IL13 il13 product has the following accession number(s) (GI #1170528) (NCBI Accession #P42203.1) (Uniprot Accession #P42203). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat IL-13 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The Picokine� Rat IL13 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat IL13 with a 96-well strip plate that is pre-coated with antibody specific for IL13. The detection antibody is a biotinylated antibody specific for IL13. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat IL13 with immunogen: Expression system for standard: E.coli; Immunogen sequence: T19-H131. The kit is analytically validated with ready to use reagents. To measure Rat IL13, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat IL13 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells\' readings against the standard curve to determine the concentration of Rat IL13 in the sample.!!Background/Introduction: Interleukin 13(IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 is cytokine secreted by many cell types, but especially T helper type 2(Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor(IL-4Ralpha) and at least one of two known IL-13-specific binding chains. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, signal transducer and activator of transcription 6(STAT6). The IL13 gene is clustered with IL3, IL5, IL4, and CSF2 on 5q. Physical maps of the 5q23-q31 region show the following order: cen--IL3--CSF2--IL13--IL4--IL5--tel, with IL13 particularly close to IL4.