|The Human. No cross reactivity with other human cytokines. No interference with soluble receptor gp80. IL-6 il6 (Catalog #MBS335703) is an ELISpot Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS335703 ELISpot Kit recognizes Human. No cross reactivity with other human cytokines. No interference with soluble receptor gp80. IL-6.
The IL-6 il6 product has the following accession number(s) (GI #7549769) (NCBI Accession #NP_036721.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Principle of the assay: A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.
Intended Uses: ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, cancerology, infectious disease, autoimmune diseases and transplantation. Utilising sandwich immuno-enzyme technology, ELISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates. Arthritis, Rheumatoid, Atherosclerosis, Autoimmune Diseases, Breast Neoplasms, Diabetes Mellitus, Type 2, Inflammation, Ischemia, Myocardial Ischemia, Necrosis, Skin Diseases are some of the diseases may be linked to Human IL-6 ELISpot Kit. Uncharacterized Tissue tissues are correlated with this protein. The following patways have been known to be associated with this gene. IL-6 also interacts with the following gene(s): Cntf, Ctf1, Fos, Il6r, Il6st, Jak1, Jak2, Jun, Nfkb1, Osm.