IL-6 elisa kit product blog
Tags: ELISA Kit; IL-6 elisa kit; IL-6;
The Human. No cross reactivity with other human cytokines. No interference with soluble receptor gp80. IL-6 il6 (Catalog #MBS335512) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS335512 ELISA Kit recognizes Human. No cross reactivity with other human cytokines. No interference with soluble receptor gp80. IL-6.The IL-6 il6 product has the following accession number(s) (GI #1161162) (NCBI Accession #AAB01430.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human IL-6 HS ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: A capture Antibody highly specific for IL-6 has been coated to the wells of the microtitre strip plate provided during manufacture. Binding of IL-6 samples and known standards to the capture antibodies and subsequent binding of the biotinylated anti-IL-6 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of IL-6 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of IL-6 in any sample tested.
Samples: Supernatants, buffered solutions or serum and plasma samples and other body fluids. This assay will recognise both natural and recombinant human IL-6.
Assay Type: Sandwich
Sensitivity: The sensitivity, minimum detectable dose of IL-6 using this IL-6 HS ELISA kit was found to be less than 0.81pg/ml. This was determined by adding 3 standard deviations to the mean OD obtained when the zero standard was assayed 40 times. Intra-assay Precision: Reproducibility within the assay was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) of 3 spiked human pooled serum samples and 2 supernatants containing different concentrations of IL-6. Data below shows the mean IL-6 concentration and the coefficient of variation for each sample. The overall intra-assay coefficient of variation has been calculated to be 4.4%.
Inter-assay Precision: Assay to assay reproducibility within one laboratory was evaluated in three independent experiments by two analysts. Each assay was carried out with 6 replicates of 3 spiked serum human pooled serum samples and 2 supernatants containing different concentrations of IL-6. The calculated overall coefficient of variation was 9.1%.