IL-5 elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; IL-5 elisa kit; IL-5;
The Mouse IL-5 il5 (Catalog #MBS355244) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS355244 ELISA Kit recognizes Mouse IL-5. MyBioSource\'s IL-5 can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA).For quantitative detection of IL-5 in mouse serum, body fluids, tissue lysate or cell culture supernate. Researchers should empirically determine the suitability of the IL-5 il5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IL-5 il5 product has the following accession number(s) (GI #54019426) (NCBI Accession #NP_068606.1) (Uniprot Accession #Q08125). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the IL-5 Mouse ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-5 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-5 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-5 can be calculated.
Background: Interleukin 5 (IL-5) is an interleukin produced by T helper-2 cells and mast cells. It is a 115-amino acid-long TH2 cytokine that is part of the hematopoietic family. It is expressed by eosinophils and has been observed in the mast cells of asthmatic airways by immunohistochemistry. IL-5 expression is regulated by several transcription factors including GATA3. IL-5 is a key mediator in eosinophil activation. It has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma, where in a large increase in the number of circulating, airway tissue, and induced sputum eosinophils have been observed. Given the high concordance of eosinophils and, in particular, allergic asthma pathology, it has been widely speculated that eosinophils have an important role in the pathology of this disease.
Assay Type: Sandwich
Detection Range: 15.6 pg/ml-1000 pg/ml
Sensitivity: < 2pg/ml.