|The Mouse IL-12p35 il12 (Catalog #MBS744303) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS744303 ELISA Kit recognizes Mouse (Murine, General) IL-12p35.
The IL-12p35 il12 product has the following accession number(s) (GI #113472827) (NCBI Accession #ABI35901.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Interleukin 12 p35 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This IL-12P35 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IL-12P35. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY IL-12P35 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-12P35 antibody and an IL-12P35-HRP conjugate. The assay sample and buffer are incubated together with IL-12P35-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-12P35 concentration since IL-12P35 from samples and IL-12P35-HRP conjugate compete for the anti-IL-12P35 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-12P35 from the sample, fewer sites are left to bind IL-12P35-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-12P35 concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 pg/mL. Mouse Interleukin 12 p35 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.