|The Mouse IgM n/a (Catalog #MBS494361) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS494361 ELISA Kit recognizes Mouse (Murine, General) IgM.
The IgM n/a product has the following accession number(s) (GI #1020017) (NCBI Accession #AAC41922.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the IgM Isotyping (Mouse) ELISA with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Description The MBS IgM Isotyping Enzyme Immunometric Assay (EIA) kit is a complete kit for the determintation of mouse IgM in Tissue Culture Media, serum and ascites fluids. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to mouse IgM immobilized on a microtiter plate to bind the mouse IgM in the standards or sample. A mouse IgM Standard is provided in the kit. After a simultaneous incubation with a polyclonal antibody to mouse IgM conjugated to Horseradish peroxidase, which binds to the mouse IgM captured on the plate, the excess reagents are washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of mouse IgM in either standards or samples. For further explanation of the principles and practices of immunoassays please see the excellent books by Chard1 or Tijssen2.
Samples: Tissue Culture Media, serum and ascites fluids
Detection Range: 0 / 3.91 - 250 ng/mL
Sensitivity: 0.60 ng/mL. Standards: 8 standards, serially diluted from 1 lyophilized standard.
Sample Volume: 50 uL
Design: Enzyme Immunometric Assay Kit
Assay Description: 1 hour (RT) + 30 min. (RT) = 1.5 hours total clock time. IgM Isotyping (Mouse) ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.