IgG3 clia kit product blog
Tags: IgG3; CLIA Kit; Human CLIA Kit; IgG3 (Immunoglobulin G3); IgG3 clia kit;
The Human IgG3 n/a (Catalog #MBS2530850) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2530850 CLIA Kit recognizes Human IgG3.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the Human IgG3 (Immunoglobulin G3) CLIA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of IgG3 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to IgG3. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IgG3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IgG3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of IgG3. You can calculate the concentration of IgG3 in the samples by comparing the RLU value of the samples to the standard curve.
Assay Type: Sandwich
Detection Range: 31.25-2000pg/mL
Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL.