IGFL3 clia kit product blog
Tags: IGFL3; CLIA Kit; IGF Like Family, Member 3 (IGFL3); IGFL3 clia kit;
The IGFL3 igfl3 (Catalog #MBS2000755) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase.The IGFL3 igfl3 product has the following accession number(s) (GI #46409418) (NCBI Accession #NP_997276.1) (Uniprot Accession #Q6UXB1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the CLIA Kit for IGF Like Family, Member 3 (IGFL3) with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate An and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the IGF Like Family, Member 3 (IGFL3) level in the sample or standard.
Assay Type: Sandwich
Samples: Tissue homogenates and other biological fluids. Detection Range: 2.743-2000pg/mL The standard curve concentrations used for the ELISA\'s were 2000pg/mL, 666.67pg/mL, 222.22pg/mL, 74.07pg/mL, 24.691pg/mL, 8.23pg/mL, 2.743pg/mL
Sensitivity: The minimum detectable dose of this kit is typically less than 11.6pg/mL
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IGF Like Family, Member 3 (IGFL3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IGF Like Family, Member 3 (IGFL3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Assay Procedure Summary1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37oC;
8. Read RLU value immediately.
Organism Species: Rattus norvegicus (Rat)
Assay Length: 4.5 hours.