|The Pig IGF2 igf2 (Catalog #MBS700241) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS700241 ELISA Kit recognizes Pig (Porcine, Swine, General) IGF2.
The IGF2 igf2 product has the following accession number(s) (GI #47523092) (NCBI Accession #NP_999048.1) (Uniprot Accession #P23695). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Pig insulin-like growth factors 2, IGF-2 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IGF-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IGF-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IGF-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Detection Range: 0.625 ng/ml-40 ng/ml
Sensitivity: 0.156 ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul
Protein Biological Process 1: Biosynthesis/Metabolism
Protein Biological Process 2: glyconeogenesis and glycometabolism
Protein Biological Process 3: Carbohydrate metabolism. Pig insulin-like growth factors 2, IGF-2 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. IGF2 also interacts with the following gene(s): GPC3, IGF1R, IGF2R, IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, IGFBP6, INSR. Blood, Bone, Brain, Connective Tissue, Embryonic Tissue, Kidney, Liver, Lung, Muscle, Placenta tissues are correlated with this protein.