IGF1 elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; IGF1 elisa kit; IGF1; IGF-1;
The Mouse IGF1 igf1 (Catalog #MBS175810) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS175810 ELISA Kit recognizes Mouse IGF1.The IGF1 igf1 product has the following accession number(s) (Uniprot Accession #E9PU89). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse IGF-1 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) (Mouse IGF-1 PicoKine ELISA Kit standard curve )
Principle of the assay: mouse IGF-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGF-1has been precoated onto 96-well plates. Standards(E.coli,G49-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-1is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGF-1 amount of sample captured in plate.
Background: Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD.1 The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A).2The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation.3 IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in aparacrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.
Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type: Sandwich
Detection Range: 62.5pg/ml-4000pg/ml
Sensitivity: < 5pg/ml.