|The Human IgE n/a (Catalog #MBS700795) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS700795 ELISA Kit recognizes Human (General) IgE.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the human Immunoglobulin E, IgE ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IgE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IgE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma
Assay Type: Sandwich
Detection Range: 62.5 ng/ml-4000 ng/ml
Sensitivity: 3.640 ng/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul
Protein Biological Process 1: Immunoglobulin. human Immunoglobulin E, IgE ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.