ICAM-1 / CD54 elisa kit product blog
Tags: ELISA Kit; Rat ELISA Kit; ICAM-1 / CD54; ICAM-1 / CD54 elisa kit;
The Rat ICAM-1 / CD54 icam1 (Catalog #MBS355378) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS355378 ELISA Kit recognizes Rat ICAM-1 / CD54. MyBioSource\'s ICAM-1 / CD54 can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA).For quantitative detection of ICAM-1 in rat serum, plasma, body fluids, tissue lysates or cell culture supernatants. Researchers should empirically determine the suitability of the ICAM-1 / CD54 icam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ICAM-1 / CD54 icam1 product has the following accession number(s) (GI #167466198) (NCBI Accession #NP_000192.2) (Uniprot Accession #P05362). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the ICAM-1 / CD54 Rat ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Background/Introduction: ICAM-1 (Intercellular Adhesion Molecule 1) also known as CD54 (Cluster of Differentiation 54) is a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by rhinovirus as a receptor. ICAM-1 is a member of the immunoglobulin superfamily, it can be induced by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFalpha) and is expressed by the vascular endothelium, macrophages, and lymphocytes.It is also an endothelial- and leukocyte-associated transmembrane protein long known for its importance in stabilizing cell-cell interactions and facilitating leukocyte endothelial transmigration. More recently, ICAM-1 has been characterized as a site for the cellular entry of human rhinovirus.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ICAM-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ICAM-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ICAM-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ICAM-1 can be calculated.
Assay Type: Sandwich
Detection Range: 31.2 pg/ml-2000 pg/ml
Sensitivity: < 2 pg/ml.