HSP-70 elisa kit product blog
Tags: ELISA Kit; Fish ELISA Kit; Heat Shock Protein 70; Hsp-70 elisa kit; Hsp-70;
The Fish HSP-70 med37d (Catalog #MBS283328) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS283328 ELISA Kit recognizes Fish HSP-70.The HSP-70 med37d product has the following accession number(s) (GI #15241847) (NCBI Accession #NP_195869.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: For the quantitative detection of Fish Growth hormone (GH) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GH. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated GH. A competitive inhibition reaction is launched between GH (Standards or samples) and biotin-conjugated GH with the pre-coated antibody specific for GH. The more amount of GH in samples, the less antibody bound by biotin-conjugated GH. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of GH in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum, Plasma And Other Biological Fluids
Assay Type: Quantitative Competitive
Detection Range: 312.5 pg/mL-5000 pg/mL
Sensitivity: 156 pg/mL (mean of 6 independent assays). Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Fish Heat Shock Protein 70 (HSP-70) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene.