|The Human hsa-mir-27a RT-PCR n/a (Catalog #MBS825429) is a PCR Kit produced from Synthetic and is intended for research purposes only. The product is available for immediate purchase. The MBS825429 PCR Kit recognizes Human (General) hsa-mir-27a RT-PCR. MyBioSource\'s hsa-mir-27a RT-PCR can be used in a range of immunoassay formats including, but not limited to, RT-PCR, Real-time RT-PCR.
The miRNAs RT-PCR primer set contains a stem-loop RT primer and a pair of specific PCR primer set. Combined with the custom\'s RT and PCR reagent. It provides a sensitive and specific method of real-time PCR for quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set. Researchers should empirically determine the suitability of the hsa-mir-27a RT-PCR n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given pcr kit. We\'ve tested the hsa-mir-27a RT-PCR Primer Set with the following immunoassay(s):
Amplication (Synthesized standard amplifiction curve (10nM/100pM))
Amplication #2 (Amplifiction curve in total RNA from 293T/A549 cells)
Dissociation Curve (Dissociation curve (Note: the minor peak on the left is the negative control))
Electrophoresis (Electrophoresis Image (20bp marker))
Stem-loop RT primer and PCR primer set to detect mature miRNA hsa-mir-27a expression.
Samples: miRNA, Total RNA
PCR Assay Type: 2-Step qRT-PCR
Detection Range: Seven orders of magnitude
Sensitivity: 10 copies of a standard mimics and as little as 25 pg of total RNA. Platform: ABI 7500 System/ViiA 7 System/StepOne/StepOnePlus/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480.