|The Human hsa-mir-10b Real-Time RT-PCR n/a (Catalog #MBS825740) is a PCR Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS825740 PCR Kit recognizes Human (General) hsa-mir-10b Real-Time RT-PCR. MyBioSource\'s hsa-mir-10b Real-Time RT-PCR can be used in a range of immunoassay formats including, but not limited to, Real-time RT-PCR.
The Real-Time RT-PCR detection and U6 calibration Kit contains the whole reagent including the stem-loop RT primer & specific PCR primer set for microRNA quantificaion and U6 primer set as housekeeping gene for calibration. It provides a sensitive and specific method of real-time PCR for relative quantificating of miRNAs from total RNA samples. It is Ideal for validating microRNA expression profiling data and high-throughput applications. Trait lies in even highly homogized miRNAs can be accurately dicriminated ensurenced by stemloop RT primer and high specific miRNAs primer set. Researchers should empirically determine the suitability of the hsa-mir-10b Real-Time RT-PCR n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given pcr kit. We\'ve tested the hsa-mir-10b Real-Time RT-PCR Detection and U6 Calibration Kit with the following immunoassay(s):
Amplication (Synthesized standard amplifiction curve (10nM/100pM))
Amplication #2 (Amplifiction curve in total RNA from 293T/A549 cells)
Dissociation Curve (Dissociation curve (Note: the minor peak on the left is the negative control))
Electrophoresis (Electrophoresis Image (20bp marker))
Detect mature miRNA hsa-mir-10b expression normalized by U6 snRNA.
Samples: miRNA, Total RNA
PCR Assay Type: 2-Step qRT-PCR
Detection Range: Seven orders of magnitude
Sensitivity: 10 copies of a standard mimics and as little as 25 pg of total RNA. Platform: ABI 7500 System/ViiA 7 System/StepOne/StepOnePlus/7900HT System; BioRad IQ5.0/CFX Connect; Stratagen MX3000p/4000p; Roche LightCycler 480
Components: 50 rxns: It contains all reagents required to perform 50 cDNA reactions and 50 PCR reactions.
100 rxns: It contains all reagents required to perform 100 cDNA reactions and 100 PCR reactions.
200 rxns: It contains all reagents required to perform 200 cDNA reactions and 200 PCR reactions.