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HP-uBAb elisa kit product blog

Posted on 2015-09-07 02:00:40 by mybiosource_staff
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Tags: ELISA Kit; Rabbit ELISA Kit; Helicobacter Pylori Urease B Antibody; HP-UBAB elisa kit; HP-UBAB;
The Rabbit HP-uBAb n/a (Catalog #MBS755188) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS755188 ELISA Kit recognizes Rabbit (General) HP-uBAb.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Helicobacter pylori urease B antibody ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) HP-uBAb.

Intended Uses: This HP-uBAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit HP-uBAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: HP-uBAb ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HP-uBAb antibody and an HP-uBAb-HRP conjugate. The assay sample and buffer are incubated together with HP-uBAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HP-uBAb concentration since HP-uBAb from samples and HP-uBAb-HRP conjugate compete for the anti-HP-uBAb antibody binding site. Since the number of sites is limited, as more sites are occupied by HP-uBAb from the sample, fewer sites are left to bind HP-uBAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HP-uBAb concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 ng/mL. Rabbit Helicobacter pylori urease B antibody ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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