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HNF4alpha elisa kit product blog

Posted on 2015-08-06 14:12:30 by mybiosource_staff
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Tags: ELISA Kit; Rabbit ELISA Kit; Hepatocyte Nuclear Factor 4 alpha; HNF4alpha elisa kit; HNF4alpha;
The Rabbit HNF4alpha hnf4a (Catalog #MBS725320) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS725320 ELISA Kit recognizes Rabbit (General) HNF4alpha.

The HNF4alpha hnf4a product has the following accession number(s) (GI #71725341) (NCBI Accession #NP_001025174.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Hepatocyte Nuclear Factor 4 Alpha ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) HNF4alpha.

Intended Uses: This HNF4a ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHNF4a in the sample, thisHNF4a ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHNF4a concentration. The concentration ofHNF4a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This HNF4a enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHNF4a. Standards or samples are then added to the microtiter plate wells andHNF4a if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHNF4a present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHNF4a are added to each well to "sandwich" theHNF4a immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, An and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containHNF4a and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Assay Type: Sandwich. Rabbit Hepatocyte Nuclear Factor 4 Alpha ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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