Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow HMGB1 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • FABP4 elisa kit blog
 • Apoh elisa kit blog
 • TACR3 elisa kit blog
 • TAF1C elisa kit blog
 • ATAD1 elisa kit blog
 • SYTL2 elisa kit blog
 • NHP2L1 elisa kit blog
 • DUOX2 elisa kit blog
 • ACTR1A elisa kit blog
 • EFTUD1 elisa kit blog
 • APLN elisa kit blog
 • SPATA16 elisa kit blog
 • AUP1 elisa kit blog
 • MTR elisa kit blog
 • DULLARD elisa kit blog
 • SBEM elisa kit blog
 • C10orf115 elisa kit blog
 • NEFH elisa kit blog
 • YPEL2 elisa kit blog
 • LRG1 elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • IFN ALPHA (Ifna15) Recombinant Protein
 • T-box transcription factor TBX20 (TBX20) Recombinant Protein
 • Putative proline iminopeptidase (pip) Recombinant Protein
 • STAT2 Antibody
 • Antitoxin RelB (relB) Recombinant Protein
 • Mannan Binding Protein (MBL2) Antibody
 • Matrix Metalloproteinase 28 (MMP-28) ELISA Kit
 • A Disintegrin And Metalloprotease 8 (ADAM8) ELISA Kit
 • DHEA 3 Antibody
 • Alanine--glyoxylate aminotransferase 2-like 1 (AGXT2L1) ELISA Kit
 • CDT1-Dup Antibody
 • sorcin (SRI) ELISA Kit
 • Insulin Like Growth Factor-I (IGF-I) Recombinant Protein
 downarrow more ...

HMGB1 elisa kit product blog

Posted on 2016-01-08 23:22:23 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Goat ELISA Kit; High Mobility Group Box1 Protein; HMGB1 elisa kit; HMGB1;
The Goat HMGB1 hmgb1 (Catalog #MBS739036) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS739036 ELISA Kit recognizes Goat (Caprine, General) HMGB1.

The HMGB1 hmgb1 product has the following accession number(s) (GI #48145843) (NCBI Accession #CAG33144.1) (Uniprot Accession #P09429). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Goat High Mobility Group box1 Protein ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) HMGB1.

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This HMGB1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat HMGB1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

PRINCIPLE OF THE ASSAY HMGB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HMGB1 antibody and an HMGB1-HRP conjugate. The assay sample and buffer are incubated together with HMGB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMGB1 concentration since HMGB1 from samples and HMGB1-HRP conjugate compete for the anti-HMGB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HMGB1 from the sample, fewer sites are left to bind HMGB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGB1 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 pg/mL. Goat High Mobility Group box1 Protein ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions