Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Assay Kit Blog  rightarrow HK1 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • IL-21 elisa kit blog
 • PADI4 elisa kit blog
 • F1+2 elisa kit blog
 • ICK elisa kit blog
 • GSTalpha5 elisa kit blog
 • C1INH elisa kit blog
 • alpha2-AP elisa kit blog
 • CD2BP2 elisa kit blog
 • ANXA9 elisa kit blog
 • LAMalpha5 elisa kit blog
 • PDK1 elisa kit blog
 • CYP7A1 elisa kit blog
 • AGAP2 elisa kit blog
 • TGFA elisa kit blog
 • CCNYL2 elisa kit blog
 • LPA elisa kit blog
 • C11orf49 elisa kit blog
 • DBF4 elisa kit blog
 • beta-Cat elisa kit blog
 • VF elisa kit blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Pulmonary surfatcant-associated protein A, SP-A ELISA Kit
 • Somatostatin (SST) Antibody
 • Defa5 Antibody
 • Sox9b Antibody
 • Helicobacter pylori, Vac Toxin Recombinant Protein
 • CD79a Antibody
 • HIVEP2 - MIBP1 Peptide
 • Lactoferrin (LTF) Antibody
 • Metalloproteinase 8 (MMP-8) ELISA Kit
 • Annexin A7 (ANXA7) Recombinant Protein
 • Adiponectin (Adipoq) Recombinant Protein
 • V5 Peptide (GKPIPNPLLGLDST) Antibody
 • Immunoglobulin M (IgM) ELISA Kit
 downarrow more ...

HK1 elisa kit product blog

Posted on 2015-11-17 11:26:10 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Porcine ELISA Kit; HK1; Hexokinase 1; HK1 elisa kit;
The Porcine HK1 hk1 (Catalog #MBS737505) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS737505 ELISA Kit recognizes Porcine (Pig, Swine, General) HK1.

The HK1 hk1 product has the following accession number(s) (GI #188497754) (NCBI Accession #NP_000179.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Porcine Hexokinase 1 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) HK1.

Intended Uses: This HK1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine HK1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: HK1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HK1 antibody and an HK1-HRP conjugate. The assay sample and buffer are incubated together with HK1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HK1 concentration since HK1 from samples and HK1-HRP conjugate compete for the anti-HK1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HK1 from the sample, fewer sites are left to bind HK1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HK1 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 1.0 U/L. Porcine Hexokinase 1 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Blood, Brain, Heart, Kidney, Liver, Lung, Muscle, Prostate, Skin, Testis tissues are correlated with this protein. The following patways have been known to be associated with this gene.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions