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HK1 clia kit product blog

Posted on 2015-11-03 21:59:15 by mybiosource_staff
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Tags: HK1; CLIA Kit; Rat CLIA Kit; HK1 (Hexokinase 1); HK1 clia kit;
The Rat HK1 hxk1 (Catalog #MBS2531736) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2531736 CLIA Kit recognizes Rat (Rattus, General) HK1.

The HK1 hxk1 product has the following accession number(s) (GI #15233457) (NCBI Accession #NP_194642.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given clia kit. We\'ve tested the Rat HK1 (Hexokinase 1) CLIA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) HK1.

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of HK1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to HK1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HK1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HK1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of HK1. You can calculate the concentration of HK1 in the samples by comparing the RLU value of the samples to the standard curve.

Assay Type: Sandwich
Detection Range: 12.5-800pg/mL
Sensitivity: Min: 7.5pg/mL; Max: 800pg/mL. Cell Culture, Flower, Other, Root, Whole Plant tissues are correlated with this protein. HK1 also interacts with the following gene(s): FBP, HKL1, PGI1, PGM2. The following patways have been known to be associated with this gene.
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