HIV elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; HIV elisa kit; HIV; HIV (Immunodeficiency Virus);
The Human HIV n/a (Catalog #MBS766122) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS766122 ELISA Kit recognizes Human HIV.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-HIV antibodies are pre-coated on onto 96-well plates. The controls, test samples and HRP- Conjugates- antibody were added to the wells subsequently, and wash with wash buffer. If any HIV (if present) are present in the sample, they will be captured inside the wells at the time of first incubation. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.
Samples: Serum, plasma and other biological fluids.
Assay Type: Qualitative. Human HIV (Human Immunodeficiency Virus) or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.